Canine Angiotensin 1-7 ELISA Kit from Innovative Research is intended for the quantitative determination of Canine Angiotensin 1-7 in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a competitive inhibition ELISA in strip well format with reagents for up to 96 tests . Ang1 present in samples reacts with an anti-Canine Ang1 monoclonal capture antibody or antigen coated and dried on a microtiter plate. Following this, Ang1 conjugated to biotin competes with the antigen in the sample to bind the capture antibody or antigen. Unbound conjugate and sample antigen is washed away and bound conjugate is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is inverse proportional to the concentration of active Ang1 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Canine Ang1. Detection Target: Canine Angiotensin 1-7 Reactivity: Canine Range: 15.6-1000pg/mL Sensitivity: 5.33pg/mL Storage Conditions: Store all components at 4┬░C This Canine Ang1 activity assay is for the quantitative determination of Angiotensin 1-7 in samples including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.