Human Adenosine A2b Receptor ELISA Kit from Innovative Research is intended for the quantitative determination of Human Adenosine A2b Receptor in biofluid samples, such as tissue homogenates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . ADORA2b present in samples reacts with an anti-Human ADORA2b capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-ADORA2b detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active ADORA2b in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human ADORA2b. Detection Target: Human Adenosine A2b Receptor Reactivity: Human Range: 0.156-10ng/mL Sensitivity: 0.061ng/mL Storage Conditions: Store all components at 4┬░C This Human ADORA2b activity assay is for the quantitative determination of Adenosine A2b Receptor in samples including tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.