Human Family With Sequence Similarity 20 Member A ELISA Kit from Innovative Research is intended for the quantitative determination of Human Family With Sequence Similarity 20, Member A in biofluid samples, such as tissue homogenates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . FAM20A present in samples reacts with an anti-Human FAM20A capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-FAM20A detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active FAM20A in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human FAM20A. Detection Target: Human Family With Sequence Similarity 20, Member A Reactivity: Human Range: 0.156-10ng/mL Sensitivity: 0.069ng/mL Storage Conditions: Store all components at 4┬░C This Human FAM20A activity assay is for the quantitative determination of Family With Sequence Similarity 20, Member A in samples including tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.