Human Fibrinogen Alpha ELISA Kit from Innovative Research is intended for the quantitative determination of Human Fibrinogen Alpha in biofluid samples, such as plasma and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . FGa present in samples reacts with an anti-Human FGa capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-FGa detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active FGa in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human FGa. Detection Target: Human Fibrinogen Alpha Reactivity: Human Range: 3.12-200ng/mL Sensitivity: 1.13ng/mL Storage Conditions: Store all components at 4┬░C This Human FGa activity assay is for the quantitative determination of Fibrinogen Alpha in samples including plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.