Human Fibrinogen Beta ELISA Kit from Innovative Research is intended for the quantitative determination of Human Fibrinogen Beta in biofluid samples, such as plasma. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . FGb present in samples reacts with an anti-Human FGb capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-FGb detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active FGb in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human FGb. Detection Target: Human Fibrinogen Beta Reactivity: Human Range: 78.1-5000ng/mL Sensitivity: 29ng/mL Storage Conditions: Store all components at 4┬░C This Human FGb activity assay is for the quantitative determination of Fibrinogen Beta in samples including plasma. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.