Human Hemoglobin Gamma 1 ELISA Kit from Innovative Research is intended for the quantitative determination of Human Hemoglobin Gamma 1 in biofluid samples, such as serum, plasma or erythrocyte lysates. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . HBg1 present in samples reacts with an anti-Human HBg1 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-HBg1 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active HBg1 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human HBg1. Detection Target: Human Hemoglobin Gamma 1 Reactivity: Human Range: 468-30000ng/mL Sensitivity: 122.6ng/mL Storage Conditions: Store all components at 4┬░C This Human HBg1 activity assay is for the quantitative determination of Hemoglobin Gamma 1 in samples including serum, plasma or erythrocyte lysates. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.