Human Histidine Decarboxylase ELISA Kit from Innovative Research is intended for the quantitative determination of Human Histidine Decarboxylase in biofluid samples, such as serum, plasma and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . HDC present in samples reacts with an anti-Human HDC capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-HDC detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active HDC in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human HDC. Detection Target: Human Histidine Decarboxylase Reactivity: Human Range: 2.34-150ng/mL Sensitivity: 0.7ng/mL Storage Conditions: Store all components at 4┬░C This Human HDC activity assay is for the quantitative determination of Histidine Decarboxylase in samples including serum, plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.